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[目的]观察复方甘正对高脂饮食诱导的大鼠非酒精性脂肪肝(NASH)的治疗作用。[方法]将30只SD大鼠随机分为3组:正常组、模型组和复方甘正治疗组。测定各组空腹血清丙氨酸氨基转移酶(ALT)、天冬氨酸转氨酶(AST)、总胆固醇(TC)、三酰甘油(TG)及肝匀浆TG、TC的水平,同时检测肝匀浆丙二醛(MDA)水平及超氧化物歧化酶(SOD)活性。[结果]模型组体重(493.20±19.55)g比正常组(371.60±31.69)g显著增高(P<0.01),治疗组(421.80±15.51)g较模型组显著降低(P<0.05)。模型组肝指数(3.33±0.74)比正常组(2.89±0.14)显著增高,治疗组(2.97±0.10)较模型组显著降低(均P<0.05);正常组肝细胞无脂肪变性,模型组普遍发生脂肪变性,治疗组肝脂肪变性程度较模型组显著减轻,正常组无炎性细胞浸润,模型组肝小叶内出现汇管区炎症,炎症活动度明显高于正常组,治疗组较模型组明显减低(均P<0.01);模型组血清ALT、AST及TC较正常组均显著升高,治疗组较模型组显著下降,但仍高于正常组(P<0.01,<0.05)。模型组血清TG水平与正常组接近,治疗组与模型组比较下降不明显;模型组肝匀浆TG、MDA水平高于正常组,SOD活性低于正常组;治疗组TG、MDA较模型组显著下降(均P<0.01),TG已降至正常水平。治疗组SOD活性较模型组明显增加,但低于正常组(均P<0.01),3组间TC水平比较差异无统计学意义。[结论]复方甘正具有显著的抗炎、抗脂质过氧化和清除氧自由基的作用。
[Objective] To observe the therapeutic effect of compound Ganzheng on non-alcoholic fatty liver (NASH) induced by high fat diet in rats. [Methods] Thirty SD rats were randomly divided into three groups: normal group, model group and compound Ganzheng treatment group. Fasting serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC), triglyceride (TG) and liver homogenate TG and TC were measured, and liver homogenate was measured simultaneously. Malondialdehyde (MDA) levels and superoxide dismutase (SOD) activity. [Results] The body weight of the model group (493.20±19.55)g was significantly higher than that of the normal group (371.60±31.69) g (P<0.01). The treatment group (421.80±15.51) g was significantly lower than the model group (P<0.05). The hepatic index (3.33±0.74) was significantly higher in the model group than in the normal group (2.89±0.14), and was significantly lower in the treatment group (2.97±0.10) than in the model group (all P<0.05). The hepatocytes in the normal group were free of steatosis, which was common in the model group. In the steatosis group, the hepatic steatosis in the treatment group was significantly less than that in the model group. There was no inflammatory cell infiltration in the normal group. In the model group, inflammation in the portal area was found in the hepatic lobule. The inflammatory activity was significantly higher in the model group than in the normal group. The treatment group was significantly lower than the model group. (P <0.01); The serum ALT, AST and TC levels in the model group were significantly higher than those in the normal group, and the treatment group was significantly lower than the model group, but still higher than the normal group (P <0.01, <0.05). The serum TG levels in the model group were similar to those in the normal group, but there was no significant difference between the treatment group and the model group. The levels of TG and MDA in liver homogenate were higher in the model group than in the normal group, and the SOD activity was lower in the model group than in the normal group; Decrease (all P<0.01), TG has dropped to normal levels. SOD activity in the treatment group was significantly higher than that in the model group, but lower than the normal group (all P<0.01). There was no significant difference in the TC level among the three groups. [Conclusion] The compound Ganzheng has significant anti-inflammatory, anti-lipid peroxidation and scavenging of oxygen free radicals.