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[目的]观察胃痞颗粒对胃黏膜上皮异型增生大鼠胃黏膜上皮细胞凋亡及调控基因蛋白表达的影响。探讨胃痞颗粒治疗胃癌前病变的作用机制。[方法]采用N-甲基-N-硝基-N-亚硝基胍(MNNG)诱导造模。设空白组、模型组、胃痞颗粒Ⅰ组及Ⅱ组,进行常规病理检测、TUNEL细胞凋亡检测、Bcl-2、Fas、P53(突变型)蛋白表达检测。[结果]胃黏膜组织病理学变化,中、重度异型增生率胃痞颗粒Ⅰ、Ⅱ组与模型组相比明显降低(均P<0.05);P53(突变型)、Bcl-2基因蛋白表达,胃痞颗粒Ⅰ、Ⅱ组明显低于模型组(均P<0.05);Fas蛋白表达,胃痞颗粒Ⅰ、Ⅱ组均高于模型组,但前者差异无统计学意义(P>0.05),后者差异有统计学意义(P<0.05)。[结论]胃痞颗粒对大鼠实验性胃黏膜癌前病变有逆转治疗作用。
[Objective] To observe the influence of Weipi Granule on the gastric mucosal epithelial cell apoptosis and the expression of regulatory genes in gastric dysplasia rats. To explore the action mechanism of Weipi granule on gastric precancerous lesions. [Method] N-methyl-N-nitro-N-nitrosoguanidine (MNNG) induced model. The blank group, model group and Weipi granule group Ⅰ and Ⅱ were set up for routine pathological examination, apoptosis detection of TUNEL cells and expression of Bcl-2, Fas, P53 (mutant) proteins. [Results] The histopathological changes of gastric mucosa, the moderate and severe dysplasia rates of Weipi Granules Ⅰ and Ⅱ groups were significantly lower than those of the model group (all P <0.05); the expressions of P53 (mutant type) and Bcl-2 protein, The expressions of Fas protein in Weipei Granules Ⅰ and Ⅱ groups were significantly lower than those in model group (P <0.05), but the former ones were not significantly different (P> 0.05) The difference was statistically significant (P <0.05). [Conclusion] Weipi granule has the effect on reversing the experimental precancerous lesions of gastric mucosa in rats.