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[目的]观察理气化痰祛瘀中药对非酒精性脂肪性肝炎(NASH)大鼠脂质过氧化反应的影响,探讨其防治NASH的作用机制。[方法]高脂饮食喂养12周诱导Wistar大鼠建立NASH模型,以不同剂量的理气化痰祛瘀中药预防及治疗,检测大鼠血清及肝组织丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)的水平,光镜观察肝组织切片病理学改变。[结果]模型大鼠肝组织出现了严重脂肪变和不同程度炎症细胞浸润。模型大鼠血及肝组织中MDA水平较正常大鼠显著升高,而SOD、GSH-PX活性则较正常大鼠显著降低。应用理气化痰祛瘀中药进行预防和治疗后,大鼠肝组织脂肪变程度和炎症活动程度较模型大鼠显著降低,同时血及肝组织MDA水平显著降低,而SOD、GSH-PX活性则显著提高。[结论]理气化痰祛瘀法中药对NASH有防治作用,其作用机制可能与抑制脂质过氧化反应有关。
[Objective] To observe the effect of traditional Chinese medicine for regulating blood circulation and removing blood stasis on lipid peroxidation in rats with non-alcoholic steatohepatitis (NASH), and to explore the mechanism of action of preventing and treating NASH. [Methods] NASH model was induced in Wistar rats induced by high-fat diet for 12 weeks. The rats were treated with different doses of qi, phlegm and blood stasis to prevent and treat them. The serum and liver tissues of rats were detected for malondialdehyde (MDA) and superoxide dismutase. (SOD) and glutathione peroxidase (GSH-PX) levels were observed under light microscopy for pathological changes in liver tissue sections. [Results] There were severe fatty changes and infiltration of inflammatory cells in different degrees in liver tissue of model rats. The levels of MDA in blood and liver tissues of model rats were significantly higher than those of normal rats, but the activities of SOD and GSH-PX were significantly lower than those of normal rats. After the application of Qi, Huayu, Chinese medicine for prevention and treatment, the degree of steatosis and inflammation in liver tissue of rats were significantly lower than those in model rats. At the same time, the levels of MDA in blood and liver tissues were significantly decreased, while the activities of SOD and GSH-PX were significantly increased. improve. [Conclusion] The traditional Chinese medicine of qi and phlegm and blood stasis has preventive and therapeutic effects on NASH, and its mechanism may be related to the inhibition of lipid peroxidation.