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本文用双盲安慰剂对照和交叉法研究前列腺素E_2同类物恩前列素(enprostil)对胃排空、口-盲肠通过时间和餐后胃肠道激素的影响。对象和方法:10名健康男性(平均24.3岁)参加研究。每名受试者给恩前列素和安慰剂各1次,其间至少间隔5天。午夜后禁食,次日晨取坐位从前臂静脉保留插管,并用含5%柠檬酸等渗盐水冲洗,保持通畅。受试者用50 ml水送服含恩前列素70μg或安慰剂的胶囊2粒,30分钟后(此时记为0分钟)进标准早餐(糖类60g、脂肪22 g和蛋白18 g,总热量530卡)。服胶囊时记为-30分钟,于-15、-5、0、15、30、60和180分钟取血标本,收集于含抑肽酶抗凝管中,保存在-20℃。血糖用氧化酶法,各激素用放免法测定。
In this paper, double-blind placebo-controlled and crossover studies were performed to investigate the effect of prostaglandin E_2 enprostil on gastric emptying, oral-cecal transit time and postprandial gastrointestinal hormones. Subjects and Methods: Ten healthy men (average 24.3 years) participated in the study. Each subject received esprostin and placebo 1 time at least 5 days apart. After midnight, fasting, the next morning to take seats from the forearm vein to retain intubation, and with 5% citric acid isotonic saline rinse, remain patency. Subjects administered 2 capsules of Enbuprost containing 70 μg of placebo or 50 mg of water, and after 30 minutes (in this case, recorded as 0 minutes), a standard breakfast (carbohydrate 60 g, fat 22 g and protein 18 g, total 530 calories). Take capsules for -30 minutes, take blood samples at -15, -5, 0, 15, 30, 60, and 180 minutes and collect in aprotinin-containing tubes and store at -20 ° C. Blood glucose with oxidase method, the determination of the various hormones by radioimmunoassay.