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目的:研究百合多糖(LP)对脂多糖(LPS)诱导的HePG2细胞增殖抑制作用的机制。方法:培养HePG2细胞,用LPS刺激8 h后加入不同浓度的百合多糖,应用MTT法检测百合多糖对HePG2细胞增殖的影响;用Western blot和细胞免疫组化检测HePG2细胞中CyclinD1和COX-2蛋白的表达;用流式细胞技术(FCM)检测细胞凋亡情况。结果:10 mg/L LPS可诱导HePG2细胞明显增殖(P<0.01),细胞中CyclinD1和COX-2表达均明显升高(P<0.01,P<0.05)。大剂量(1 g/L)、中剂量(0.5 g/L)百合多糖干预后,明显抑制LPS诱导的HePG2细胞增殖(P<0.01),促使HePG2细胞G0/G1比例明显增加,细胞中CyclinD1明显降低(P<0.01,P<0.05),而对COX-2无影响。结论:百合多糖可能通过降低CyclinD1的表达抑制HePG2细胞增殖。
AIM: To investigate the mechanism of Lilium polysaccharide (LP) inhibiting the proliferation of HepG2 cells induced by lipopolysaccharide (LPS). Methods: HePG2 cells were cultured and treated with LPS for 8 h, then different concentrations of lily polysaccharides were added. The effects of lily polysaccharide on the proliferation of HepG2 cells were detected by MTT assay. The expressions of CyclinD1 and COX-2 protein in HepG2 cells were detected by Western blot and immunohistochemistry The apoptosis was detected by flow cytometry (FCM). Results: The proliferation of HepG2 cells was induced by 10 mg / L LPS (P <0.01). The expressions of CyclinD1 and COX-2 were significantly increased (P <0.01, P <0.05). LPS-induced LPS-induced HepG2 cells proliferation (P <0.01) after induced by high dose (1 g / L) and medium dose (0.5 g / L) lilium polysaccharide significantly increased G0 / G1 ratio of HepG2 cells and significantly increased CyclinD1 (P <0.01, P <0.05), but had no effect on COX-2. Conclusion: Lily polysaccharide may inhibit HepG2 cell proliferation by decreasing the expression of CyclinD1.