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神经降压素(neurotensin,NT)是1973年由Garranway和Leeman等发现的一种脑肠肽,为13个氨基酸构成的单链多肽,分子量1674,由N细胞分泌,N细胞广泛分布于脑和胃肠道中。本文测定了肝硬化及胃、十二指肠溃疡患者血浆中NT的含量,旨在探讨NT在上述疾病发病机理中的作用。 对象和方法 一、对象:选择本院健康工作人员30例(男20,女10),作为对照组,年龄24～60岁,平均41.1岁。选择诊断肯定的肝炎后肝硬化患者30例(男22,女8),年龄31～58岁,平均45.7岁。经胃镜和病理证实的胃溃疡病人31例(男24,女7)年龄19～57岁,平均35.9岁。十二指肠球部溃疡患者32例(男23,女9),年龄20～60岁,平均38.2岁。 二、方法:取受检者肘前静脉血5ml注入含40μl10％的EDTA-Na_2和60μl抑肽酶的抗凝管中,混匀置4℃3000rpm离心10min,分离血浆,置-30℃保存,测定前使样品置于室温复融,再次4℃3500rpm离心,取上清液测定。NT RIA kit 由北京解放军东亚免疫技术研究所提供,按说明书操作。采用北京262厂生产的F J-2008G型自动γ免疫计数器测定。数据经统计学处理采用均数±标准差表示,组间差异显著性用t检验分析。
Neurotensin (NT) is a brain-gut peptide discovered by Garranway and Leeman in 1973. It is a 13-amino acid single-chain polypeptide with a molecular weight of 1674. It is secreted by N-cells and N-cells are widely distributed in the brain and In the gastrointestinal tract. This article measured the liver cirrhosis and gastric, duodenal ulcer in patients with plasma NT content, to explore the role of NT in the pathogenesis of the above diseases. Subjects and methods A, object: Select our hospital health workers in 30 cases (20 men and 10 women), as a control group, aged 24 to 60 years, mean 41.1 years. Select the diagnosis of positive hepatitis cirrhosis in patients with 30 cases (male 22, female 8), aged 31 to 58 years, mean 45.7 years. 31 cases of gastric ulcer confirmed by endoscopy and pathology (male 24, female 7) aged 19 to 57 years old, with an average of 35.9 years old. Duodenal ulcer patients 32 (male 23, female 9), aged 20 to 60 years, mean 38.2 years. Second, the method: take subjects elbow venous blood 5ml injected 40μl10% EDTA-Na_2 and 60μl aprotinin anticoagulant tube, mix well and centrifuged at 3000rpm for 10min at 4 ℃, the plasma was separated and stored at -30 ℃, Before the measurement, the sample was allowed to thaw at room temperature, centrifuged again at 3500 rpm at 4 ° C, and the supernatant was measured. NT RIA kit by the Beijing East Asian Institute of Immunization Technology, provided by the manual. FJ-2008G automatic γ immunization counter measured by Beijing 262 Plant was used. Data were statistically treated with mean ± SD, significant differences between groups using t test analysis.