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GB病毒C(GBV-C)是新近发现的感染人类的黄病毒,可能与人类肝病有关。从发现至今,GBV-C的检测一直依赖于RT-PCR技术,这需要病人血清中存在足量的病毒基因组RNA。对于血清中病毒RNA量不足者,如病人已处于恢复期或病毒RNA已被(或近)清除者,则不适合。这样的病例多已产生血清抗体。所以,这时对血清抗体的检测尤为重要。但由于GBV-C还不能在细胞培养中生长,因而尚无病毒自身的抗原可供血清学检测用。已有的、原核系统中表达的重组病毒抗原蛋白也因其抗原性不强,未能
GB virus C (GBV-C) is a newly discovered human-caused flavivirus that may be associated with human liver disease. Since its discovery, detection of GBV-C has relied on RT-PCR technology, which requires the presence of a sufficient amount of viral genomic RNA in the patient’s serum. Not suitable for serum underexploited RNA if the patient is in convalescence or if the viral RNA has been (or is) cleared. Many cases have produced serum antibodies. So, then the detection of serum antibodies is particularly important. However, since GBV-C is not yet able to grow in cell culture, there is no viral antigens available for serological testing. Existing, prokaryotic expression of recombinant viral antigen protein because of its antigenicity is not strong, failed to