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目的探讨小窝蛋白(Cav)-1在食管癌细胞迁移中的作用,并探讨两者之间的关系。方法体外培养人食管癌TE13细胞株,采用Cav-1小干扰RNA(siRNA)转染TE13细胞(转染组),以未转染细胞为对照(NC)组。采用蛋白免疫印迹(Western blot)实验检测细胞的Cav-1表达水平,采用划痕实验观察细胞迁移情况,探讨Cav-1和食管癌细胞迁移的关系。结果 Western blot实验结果显示,食管癌TE13细胞高表达Cav-1,转染siRNA后细胞Cav-1表达水平较转染前明显下降,转染组和未转染组细胞的灰度值分别为(1.98±0.34)和(3.15±6.10),两者比较差异有统计学意义(t=4.26,P<0.05)。划痕试验结果显示,未转染组在划痕后24 h痕间距明显缩窄,部分痕区域细胞密集;而转染组细胞在划痕后24 h痕间距未见明显缩窄。表明转染后细胞的迁移能力较转染前明显下降。结论 Cav-1在食管癌的发生、发展过程中扮演着促癌基因的角色,抑制Cav-1表达可能抑制食管癌的进展。
Objective To investigate the role of caveolin-1 (Cav-1) in esophageal cancer cell migration and to explore the relationship between the two. Methods Human esophageal cancer TE13 cell line was cultured in vitro. TE13 cells (transfected group) were transfected with Cav-1 siRNA. The untransfected cells were used as the control group (NC). Western blot was used to detect the expression of Cav-1 in cells. Scratch assay was used to observe the migration of Cav-1 and esophageal cancer cells. Results The results of Western blot showed that Cav-1 was highly expressed in esophageal cancer TE13 cells and the expression of Cav-1 was significantly decreased after transfection compared with that before transfection. The gray values of cells in transfected and untransfected groups were ( 1.98 ± 0.34) and (3.15 ± 6.10), respectively. The difference between the two groups was statistically significant (t = 4.26, P <0.05). Scratch test results showed that the non-transfected group 24 hours after scratching marks significantly narrowed the spacing, some of the scar-area cells dense; and transfected cells within 24 h after scratching no significant narrowing of the distance between the marks. The results showed that the transfection ability of cells after transfection was significantly lower than that before transfection. Conclusions Cav-1 plays a role of oncogene in the development and progression of esophageal cancer. Suppression of Cav-1 expression may inhibit the progression of esophageal cancer.