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本文探索出一种用碗豆（ＰｉｓｕｍｓａｔｉｖｕｍＬ．）和狭叶羽扇豆（ＬｕｐｉｎｕｓａｎｇｕｓｔｉｆｏｌｉｕｓＬ．）的根对磺酰脲类（绿磺隆、ｔｒｉａｓｕｌｆｕｒｏｎ和甲磺隆）与三唑嘧啶磺酰胺类（ｆｌｕｍｅｔｓｕｌａｍ和ｍｅｔｏ－ｓｕｌａｍ）除草剂进行常规评估的生物测定方法。对ｐＨ值从５．８至８．４，质地从砂土至粘土的土壤进行了试验。两种植物对绿磺隆和ｆｌｕｍｅｔｓｕｌａｍ的灵敏度相似，它们对不同除草剂和不同土壤类型的反应范围分别为：０．７５～６．０ｎｇｔｒｉａｓｕｌｆｕｒｏｎ／ｇ土壤（Ｗｉｍｍｅｒａ灰粘土），０．１２５～８．０ｎｇ绿磺隆／ｇ土壤（Ｍａｌｅｅ砂土）。这种方法具有广泛的用途，包括绿磺隆和ｆｕｌｍｅｔｓｕｌａｍ制剂的初始生物活性测定，ｔｒｉａｓｕｌｆｕｒｏｎ田间淋溶与持留的检测，以及用甲磺隆和ｍｅｔｏｓｕｌａｍ测量两种类型除草剂之间相对功效。本生物测定反应具有较高的重复性和精确度，可通过逻辑斯谛拟合曲线程序计算。在每一种情况下，Ｒ２值均大于０．９０，在０．０５水平，拟合度检验结果为不显著，并用卡方测验了两种类型除草剂的ＥＤ５０间的差异。本方法不需进行催芽、种苗选择、日常灌水或洗根等处理，播种后７ｄ可得?
In this paper, we explored a method for the determination of sulfonylureas (triflusulfuron, triasulfuron and metsulfuron-methyl) and trimethoprim sulfonamides (flumetsulam and metozole) with the roots of Pisum sativum L. And Lupinus angustifolius L., -sulam) herbicides for routine assessment of bioassay methods. Soil samples ranging from sand to clay were tested for soil pH from 5.8 to 8.4. The sensitivities of the two plants to chlorsulfuron and flumetsulam were similar and their response ranges for different herbicides and different soil types were: 0.75 to 6.0 ngtriasulfuron / g soil (Wimmera limestone), 0.125 to 8, respectively. 0 ng chlorsulfuron / g soil (Malee sand). This method has a wide range of uses, including the initial bioassay of the chlorsulfuron and fulmetsulam formulations, the detection of leaching and retention of triasulfuron in the field, and the relative efficacy of the two types of herbicides with metsulfuron and metosulam. The bioassay response is highly repeatable and accurate and can be calculated using logistic curve fitting programs. In each case, R2 values were greater than 0.90, and at 0.05, the fit test was insignificant and the ED50 differences between the two types of herbicides were tested using chi-square test. This method does not require germination, seed selection, daily irrigation or root washing and other treatment, 7d after sowing available?