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用两种实验模型观察三硝基甲苯(TNT)对大鼠肝细胞质膜Ca~(2+)-ATP酶和蛋白巯基含量的影响。结果表明:当TNT直接与Percoll密度梯度离心分离的质膜及NADPH在37℃温育30或60min后,上述两项指标均未见明显改变;但若将TNT与胶原灌注分离的肝细胞在37℃温育同样的时间,然后用Affi-Gel 731聚阳离子凝胶颗粒吸附法分离质膜,则质膜Ca~(2+)-ATP酶活性和蛋白巯基含量均见明显下降,且呈剂量时间效应关系,两项观察指标之间密切相关。若在肝细胞的温育介质中加入二硫苏糖醇或GSH,则前者对Ca~(2+)-ATP酶活性具有明显的保护作用,而GSH作用不明显。
Two experimental models were used to observe the effects of TNT on the plasma membrane Ca ~ (2 +) - ATPase and protein sulfhydryl content of rat liver cells. The results showed that when TNT was directly separated from Percoll density gradient centrifugation plasma membrane and NADPH incubated at 37 ℃ for 30 or 60min, there was no significant change in the above two indexes; however, if the TNT and collagen perfusion isolated hepatocytes 37 Then the plasma membrane was separated by Affi-Gel 731 polycation gel particle adsorption method, the Ca (2 +) - ATPase activity of plasma membrane and the content of protein sulfhydryl group were significantly decreased, and the dose time The effect of the relationship between the two indicators are closely related. If dithiothreitol or GSH was added into the incubation medium of hepatocytes, the former had a significant protective effect on Ca ~ (2 +) - ATPase activity, while the effect of GSH was not obvious.