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Objective:To investigate the role of water-soluble extract of Salvia fruticosa(Creek sage)(S.fruticosa) leaves in reducing both intrinsic cellular and H_2O_2-induced DNA oxidation in cultured human embryonic kidney 293 cells.S.fruiicosa.native to the Eastern-Mediterranean basin,is widely used as a medicinal herb for treatment of various diseases.Methods:Dried leaves of 5.fruticosa were extracted in phosphate buffer saline and purified using both vacuum and high pressure filtrations.Each mL of the preparation contained(7.1±1.0)mg of extract.HEK-293 cells were incubated in one set with S.fruticosa extract in the presence of 0.1 mmol/L H_2O_2,and in the other set with the addition of the extract alone.The DNA oxidation was measured using fluorescence upon fluorescein isothiocyanate derivarization of 8-oxoguanine moieties.The fluorescence was measured using flow cytometry technique.Results:Cells incubated 3 h with 150μL extract and exposed to 0.1 mmol/L H_2O_2 showed lower intensity of fluorescence,and thus lower DNA oxidation.Moreover,cells incubated 3 h with 100μl.of the extract showed lower intensity of fluorescence,and thus lower intrinsic cellular DNA oxidation compared to control(without S.fruticosa).Conchisions:The results from this study suggest that the water-soluble extract of S.fruticosa leaves protects against both H_2O_2-induced and intrinsic cellular DNA oxidation in human embryonic kidney 293 cells.
Objective: To investigate the role of water-soluble extract of Salvia fruticosa (Creek sage) (S. fruticosa) leaves in reducing both intrinsic cellular and H 2 O 2 -induced DNA oxidation in cultured human embryonic kidney 293 cells S. fruiicosa. Native to the Eastern-Mediterranean basin, is widely used as a medicinal herb for treatment of various diseases. Methods: Dried leaves of 5.fruticosa were extracted in phosphate buffer saline and purified using both vacuum and high pressure filtrations .ach mL of the preparation contained (7.1 ± 1.0) mg of extract. HEK-293 cells were incubated in one set with S.fruticosa extract in the presence of 0.1 mmol / L H 2 O 2, and in the other set with the addition of the extract alone. The DNA oxidation was measured using fluorescence upon fluorescein isothiocyanate derivarization of 8-oxoguanine moieties. The fluorescence was measured using flow cytometry technique. Results: Cells incubated 3 h with 150 μL extract and exposed to 0.1 mmol / L H_2O_2 showed lower intensity of f luorescence, and thus lower DNA oxidation. Moreover, cells incubated 3 h with 100 μl. of the extract showed lower intensity of fluorescence, and thus lower intrinsic cellular DNA oxidation compared to control (without S.fruticosa) .Conchisions: The results from this study suggest that the water-soluble extract of S.fruticosa leaves protects against both H 2 O 2 -induced and intrinsic cellular DNA oxidation in human embryonic kidney 293 cells.