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目的:通过建立后肢缺血预适应大鼠模型,研究远程预适应对大鼠肾脏缺血-再灌注损伤保护作用的机制。方法:48只健康雄性SD大鼠摘除右肾后随机分为4组:假手术组(sham组)、单纯后肢预适应组(LIP组)、单纯肾缺血再灌注组(IR组)、后肢预适应+肾缺血组(LIP-IR组),再灌注24 h后检测血肌酐、尿素氮水平,肾组织病理学改变,肾组织中一氧化氮(NO)及一氧化氮合酶(NOS)活性,包括总NOS(TNOS)、诱导型NOS(iNOS)和结构型NOS(cNOS)。结果:与IR组相比,LIP-IR组血肌酐、尿素氮、肾小管评分,肾组织中TNOS、iNOS活性均明显降低,但肾组织NO、cNOS活性明显增高(P<0.05);LIP组与sham组相比,上述指标无显著性差异(P>0.05)。结论:肢体缺血预适应能够减轻大鼠肾脏缺血-再灌注损伤,其可能与LIP上调eNOS表达,同时降低肾脏iNOS而发挥肾保护作用有关。
OBJECTIVE: To establish a rat model of hindlimb ischemic preconditioning to study the protective effect of remote preconditioning on renal ischemia-reperfusion injury in rats. Methods: Forty eight healthy male SD rats were randomly divided into 4 groups: Sham group, LIP group, IR group, hindlimb group Pretreatment + renal ischemia group (LIP-IR group), 24 h after reperfusion serum creatinine, blood urea nitrogen levels, pathological changes of renal tissue, renal tissue nitric oxide (NO) and nitric oxide synthase (NOS ) Activity including total NOS (TNOS), inducible NOS (iNOS) and constitutive NOS (cNOS). Results: Serum creatinine, urea nitrogen, tubule score, TNOS and iNOS activity in LIP-IR group were significantly lower than those in IR group, but NO and cNOS activity in renal tissue were significantly increased (P <0.05); LIP group Compared with sham group, there was no significant difference between the above indexes (P> 0.05). Conclusion: Limb ischemic preconditioning can reduce renal ischemia-reperfusion injury in rats, which may be related to LIP upregulation of eNOS expression, renal iNOS reduction and renal protection.