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目的:阐明前列腺素E2(prostaglandin E2,PGE2)通过EP1受体上调肝癌细胞Huh-7中β1-integrin的表达及其相关的信号转导通路.方法:用PGE2、EP1受体激动剂(17-PT-PGE2)、EP1受体抑制剂SC19220、NF-κB抑制剂PDTC处理Huh-7细胞,通过Western blot、免疫荧光组织化学实验等方法检测β1-integrin蛋白表达水平和NF-κB的活性.结果:5μmol/L的PGE2处理Huh-7细胞24 h后,β1-integrin的表达水平与对照组相比上升了129.48%(P<0.01),5μmol/L EP1受体激动剂17-PT-PGE2处理使细胞β1-integrin的蛋白表达水平升高了216.34%(P<0.01).10μmol/L的EP1受体抑制剂SC19220处理后β1-integrin表达水平与PGE2组相比下降了34.51%(P<0.05).免疫荧光组织化学实验显示17-PT-PGE2处理Huh-7细胞120 min后,NF-κB核表达水平明显增加;Western blot实验显示5μmol/L 17-PT-PGE2处理Huh-7细胞120 min后,磷酸化NF-κB-p65上升了209.27%(P<0.01).NF-κB抑制剂PDTC处理Huh-7细胞后β1-integrin蛋白表达水平与EP1受体激动剂组相比降低了63.49%(P<0.01).结论:PGE2可通过EP1受体上调Huh-7细胞中β1-integrin的表达,此调节作用可能与NF-κB信号转导通路有关.“,”Objective:To investigate the effect of prostaglandin E2(PGE2) on the expression of β1-integrin and its related signaling pathway in Huh-7 cells.Methods:Huh-7 cells were treated with PGE2,EP1 receptor agonist(17-phenyltrinor Prostaglandin E2,17-PTPGE2),EP1 receptor antagonist SC19220,nuclear factor-κappaB(NF-κB)inhibitor PDTC.Western blot and immunofluorescence test were employed to detect the expression of β1-integrin and activation of NF-κB in Huh-7 cells.Results:When Huh-7 cells were treated with 5 μmol/L PGE2 for 24 h,the level of β1-integrin was increased by 129.48%(P<0.01).EP1 receptor agonist(5 μmol/L 17-PT-PGE2) mimicked the effect of PGE2,and increased the expression of β1-integrin by 216.34%(P<0.01).EP1 receptor antagonist SC19220(10 μmol/L) suppressed PGE2-mediated expression of β1-integrin by 34.51%(P<0.05).In immunofluorescence assays,NF-κB translocated into the nucleus induced by 17-PT-PGE2 in Huh-7 cells.Further,Western blot assays showed that the level of Phospho-NF-κB-P65 was increased by 209.27%(P<0.01)after treatment of 5 μmol/L 17-PT-PGE2 for 120 min.NF-κB inhibitor PDTC decreased EP1-mediated expression of β1-integrin by 63.49%(P<0.01).Conclusion:PGE2 might up-regulate the expression level of β1-integrin through EP1 receptor in Huh-7 cells,which was partly related to the NF-κB signaling pathway.