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目的:测定尘肺病患者外周血中SDC-2基因的表达水平,探讨检测该指标作为尘肺病相关生物标志物的可行性。方法:分别用酶联免疫吸附试验(ELISA)和实时定量RT-PCR方法,对77例尘肺病患者和162例健康体检者外周血中SDC-2基因的表达变化进行测定。结果:尘肺病患者血清SDC-2的浓度为42.37±19.32 pg/ml,健康对照组为37.08±6.24 pg/ml,两组之间的差异没有统计学意义(F=3.278,P=0.074);矽肺患者血清SDC-2的浓度水平高于煤工尘肺患者,但两者未有统计学差异(F=0.803,P=0.377);不同影像分期尘肺病患者血清中SDC-2的浓度水平差异无统计学意义(P>0.05);尘肺病患者肺功能损害程度与血清SDC-2浓度没有相关性(r=-0.159,P=0.361);SDC-2基因转录水平的变化趋势与其血清浓度的变化趋势基本一致。结论:SDC-2基因的表达变化作为尘肺病相关血液学生物标志物可能是不可行的。
Objective: To determine the expression level of SDC-2 gene in peripheral blood of patients with pneumoconiosis and to explore the feasibility of detecting this index as a biomarker of pneumoconiosis. Methods: The changes of SDC-2 gene expression in peripheral blood of 77 patients with pneumoconiosis and 162 healthy controls were detected by enzyme-linked immunosorbent assay (ELISA) and real-time quantitative RT-PCR. Results: The serum concentration of SDC-2 in patients with pneumoconiosis was 42.37 ± 19.32 pg / ml and that in healthy controls was 37.08 ± 6.24 pg / ml. There was no significant difference between the two groups (F = 3.278, P = 0.074). The serum concentration of SDC-2 in patients with silicosis was higher than that in coal workers with pneumoconiosis, but there was no significant difference between them (F = 0.803, P = 0.377). The difference of SDC-2 levels in patients with different stages of pneumoconiosis (R = -0.159, P = 0.361). The change trend of SDC-2 gene transcription level and the change of serum concentration of SDC-2 in patients with pneumoconiosis had no significant difference The trend is basically the same. Conclusions: Changes in the expression of the SDC-2 gene may not be feasible as a hematological biomarker associated with pneumoconiosis.