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目的 探讨Ki 67、端粒酶在正常结直肠粘膜、结直肠良、恶性病变中的表达及其临床意义。方法 流式细胞仪测定 41例结直肠癌、9例结直肠良性病变和 1 3例正常结直肠粘膜标本的Ki 67标记指数 ,半定量端粒重复扩增 (TRAP) 银染法测定其端粒酶活性 ,并行统计学处理。结果 正常结直肠粘膜、良、恶性病变的Ki 67标记指数与端粒酶相对值的组间比较均有显著性差异 (P <0 .0 5) ;结直肠癌病人的两指标在各临床资料的组间比较中均无显著性差异 (P >0 .0 5) ;两者联合检测鉴别结直肠良、恶性病变的灵敏度、特异性分别为 80 .49%、77.78% ;结直肠癌病人两指标线性相关系数为 0 .852 (P <0 .0 5)。结论 Ki 67表达、端粒酶活性是鉴别正常结直肠粘膜、结直肠良、恶性病变有价值的两项指标 ,但端粒酶活性鉴别结直肠良、恶性病变的灵敏度不理想 ;两指标与结直肠癌的一些临床指标无关 ;结直肠癌病人的两指标间存在线性相关关系。
Objective To investigate the expression and clinical significance of Ki 67 in human benign and malignant colorectal mucosa and normal colorectal mucosa. Methods The Ki 67 labeling index of 41 colorectal carcinomas, 9 colorectal benign lesions and 13 normal colorectal mucosa specimens were determined by flow cytometry. The telomeres were determined by semi-quantitative telomeric repeat amplification (TRAP) Enzyme activity, parallel statistical analysis. Results There was a significant difference between the groups of Ki 67 labeling index and telomerase relative value in normal colorectal mucosa, benign and malignant lesions (P <0.05). The two indexes in patients with colorectal cancer were significantly different in all clinical data There was no significant difference between the two groups (P> 0.05). The sensitivity and specificity of the two methods for detecting benign and malignant colorectal lesions were 80.49% and 77.78% The linear correlation coefficient was 0.852 (P <0.05). Conclusions The expression of Ki 67 and telomerase activity are two valuable indexes for differentiating benign and malignant colorectal mucosa from colorectal mucosa. However, the sensitivity of telomerase activity in differentiating benign and malignant colorectal lesions is not satisfactory. Some clinical indicators of rectal cancer has nothing to do; there is a linear correlation between the two indicators of patients with colorectal cancer.