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目的:探讨雌激素在脓毒症大鼠骨骼肌蛋白高分解代谢中的作用及其可能机制。方法:采用腹腔内注射10 mg/kg内毒素制造脓毒症动物模型。将36只大鼠随机分为正常对照组、脓毒症组、脓毒症+雌激素注射组,每组12只。采用高效液相色谱分析法测定酪氨酸和三甲基组氨酸,实时聚合酶链式反应(RT-PCR)分析肌肉和下丘脑组织中白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)、骨骼肌萎缩基因(MuRF-1)、雌激素受体α(ER-α)和雌激素受体β(ER-β)mRNA的表达。结果:脓毒症组大鼠三甲基组氨酸(3-MH)、酪氨酸(Tyr)水平和萎缩基因MuRF-1 mRNA表达上调(P<0.05),而且中枢IL-1βmRNA和TNF-αmRNA表达量增加(P<0.05),ER-α、ER-βmRNA表达量减少(P<0.05)。给予皮下注射雌激素后,外周骨骼肌消耗及中枢炎症减轻,且有显著性差异(P<0.05)。下丘脑中雌激素受体表达与炎性递质表达呈负相关(P<0.05)。结论:雌激素能缓解骨骼肌高分解代谢,可能与其能减轻下丘脑炎症有关。
Objective: To investigate the role of estrogen in skeletal muscle protein catabolism in septic rats and its possible mechanism. Methods: The model of sepsis was made by intraperitoneal injection of 10 mg / kg endotoxin. 36 rats were randomly divided into normal control group, sepsis group, sepsis + estrogen injection group, 12 rats in each group. The contents of IL-1β, IL-1β in muscle and hypothalamus were determined by real-time polymerase chain reaction (RT-PCR) using high performance liquid chromatographic method for the determination of tyrosine and trimethyl histidine. α, TNF-α, MuRF-1, ER-α and ER-β mRNA. Results: The levels of 3-MH, Tyr and MuRF-1 mRNA in sepsis group were up-regulated (P <0.05), and the levels of IL-1βmRNA and TNF- α mRNA expression increased (P <0.05), ER-α, ER-βmRNA expression decreased (P <0.05). After administration of estrogen subcutaneously, peripheral skeletal muscle consumption and central inflammation were relieved, with significant difference (P <0.05). There was a negative correlation between the expression of estrogen receptor and inflammatory mediators in the hypothalamus (P <0.05). Conclusion: Estrogen can relieve skeletal muscle hypermetabolism, which may be related to its ability to relieve hypothalamic inflammation.