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应用纯化的ＨＥＶ（８７Ａ株）热灭活抗原进行ＥＬＩＳＡ试验，测定日本大阪地区收集的１４９例各种肝病患者急性期血清中的抗ＨＥＶ抗休，获得１９份阳性（阳性率为１２．７％）。其中单纯抗ＨＥＶＩｇＭ阳性１１份和抗ＨＥＶＩｇＧ阳性６份，两者都阳性的２份。同时测定３０７例健康人体检标本，获得单纯抗ＨＥＶＩｇＭ和ＩｇＧ阳性各１份（阳性率为０．６％）。表明日本人群中存在ＨＥＶ感染
Anti-HEV (87A strain) inactivated antigens were tested by ELISA. The anti-HEV activity of 149 patients with various liver diseases collected from Osaka, Japan in the acute phase was determined and 19 positive (positive rate was 12.7% ). Among them, 11 were anti-HEVIgM-positive and 6-anti-HEVIgG were positive, two were positive. At the same time, 307 healthy subjects were tested and 1 anti-HEVIgM and IgG positive (0.6% positive) were obtained. This indicates that HEV infection exists in the Japanese population