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收集豆科SSR引物,通过分子标记技术,筛选验证可用于黄芪与红芪鉴定的核心引物。结果表明,101对SSR引物中筛选出6对有效鉴定引物,其PCR产物在相对分子质量100~500 bp,可形成的7~12条数目不等的电泳条带,其中多态性位点55个,多态性比率为100%,平均多态信息含量为0.371,多态位点建立的聚类分析(UPMGA)结果显示,获得的核心引物可在相似度为0.4处100%区分62份黄芪与红芪的混合样品,标记参试引物及对应的特征泳带,生成指纹图谱代码,为黄芪与红芪的鉴别提供依据。
The legume SSR primers were collected and screened to validate the core primers that can be used for identification of Radix Astragali and Radix Astragali by molecular marker technology. The results showed that sixty pairs of 101 SSR primers were screened and their PCR products were in the range of 100 ~ 500 bp with a molecular weight of 7 ~ 12. The polymorphic loci 55 The polymorphic loci were clustered (UPMGA). The results of UPMGA showed that the obtained primers could differentiate 62 parts of Astragalus mongholicus at a similarity of 0.4% And the mixed sample of Radix Hedysari, mark the primer of the primer and the corresponding characteristic band, and generate the fingerprint code to provide the basis for the identification of Radix Astragali and Radix Astragali.