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Objective:
1.To determine the cardiac structural changes and molecular events involving in Myocardial Remodeling and Fibrosis during sustained pressure-overload hypertrophy in Rats.
2.To determine the effects of Fuzheng Huayu (FZHY) capsule in improving myocardial fibrosis and remodeling following sustained pressure-overload hypertrophy in Rats.
Method:
1.To make sustained pressure-overload cardiac hypertrophy in rats,constriction of suprarenal abdominal aorta was performed in rats in order to make model,then to compare the heart structural changes of eight weeks and four weeks model rats, using echocardiographic techniques,and then parameters involving in myocardial fibrosis including CollagenⅠ, CollagenⅢ, TGF-β1 mRNA gene
expression,and TGF-β1 protein of 4 weeks and 8 weeks model rats were evaluated using pathologic techniques, Gene Chip and confirming through Realtime PCR.
2.To study the efficacy of Fuzheng huayu (FZHY) capsule in left ventricular remodeling and
fibrosis induced by Constriction of abdominal aorta, the rats were divided into four groups including model group, sham-operated group, Captopril group, and Fuzhenghuayu capsule group, then evaluating the parameters involving in myocardial fibrosis including heart structure, CollagenⅠ, Collagen Ⅲ,TGF-β1 mRNA gene expression, and TGF-β1 protein of 4 weeks and 8 weeks model rats using pathologic techniques, Gene Chip and confirming through Realtime PCR of eight weeks and four weeks rats.
Conclusion:
1.Constriction of suprarenal of abdominal aorta can induce characteristics of concentric hypertrophy and Myocardial Fibrosis eight weeks after operation on Rats.
2.Fuzheng Huayu can improve concentric hypertrophy or myocardial fibrosis of model rats.
3.Fuzheng Huayu can reduce TGF-β1mRNA Gene Expression in myocardial fibrosis in model rats.