Molecular Cloning and Characterization of Vacuolar Na+H+ Antiporter from Nitraria sibirica Pall

来源 :2rd International Symposium on Integrative Plant Biology第二届国 | 被引量 : 0次 | 上传用户:dyjianing88
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  The Nitraria L.is a genus of flowering plants in the Zygophyllaceae family, which grows primarily in deserts and semideserts, and exhibits a strong salt-resistance.The Nitraria plants have important ecological and economic value, therefore, research on the molecular mechanisms of salt tolerance is very necessary in Nitraria.Vacuolar Na+/H+, antiporters play an important role in plant salt tolerance, which can deliver Na+ into the vacuoles against the electrochemical gradient.The compartmentation of Na+ provides an effective mechanism to avert the deleterious effects of Na+ on cytosol, and maintain an osmotic potential by Na+ storage in the vacuoles.In the study, using degenerate PCR and the rapid amplification of cDNA ends (RACE) method, a Na+/H+antiporter gene (referred to as NsNHX1) was isolated from Nitraria sibirica Pall.
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