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The cortical endoplasmic reticulum (ER) network in plant cells is a highly dynamic structure; its movements are regulated by the actin cytoskeleton in most cell types.Therefore, there must be mechanisms that hold the ER network in position while it is remodelling.Two recent studies on ER persistency mapping and optical laser trapping indicated the existence of ER-to-plasma membrane-(PM)-anchoring sites.To date, proteins that are targeted to the ER/PM anchor sites or regulated ER anchoring have not been identified.Here, we show that the family of proteins, VAP (vesicle-associated membrane protein-associated protein), may be involved in this process.In both mammal and plants, VAPs have been shown to be involved in lipid transport.In our study, two proteins within this family, PVA12 and VAP27 were studied and characterised using fluorescent protein fusions and confocal microscopy in both tobacco and Arabidopsis.