【摘 要】
：
The nuclear transcription factor signal transducer and activator of transcription 3 (STAT3) plays a significant role in cell cycling and proliferation.STAT3 is activated via phosphorylation by Janus k
【机 构】
：
Department of Physiology and Pathophysiology,Shanghai Medical College,Fudan University,Shanghai 2000
【出 处】
：
International Conference for Physiological Sciences 2012(201
论文部分内容阅读
The nuclear transcription factor signal transducer and activator of transcription 3 (STAT3) plays a significant role in cell cycling and proliferation.STAT3 is activated via phosphorylation by Janus kinase2 (JAK2)and acetylation by histone acetyltransferase p300/CBR It was recently noticed that STAT3 signaling pathway was involved in regulating the genes that contribute to renal interstitial fibrosis.Previous studies have demonstrated that tyrosine kinases mediate angiotensin Ⅱ (Ang Ⅱ) effects in different cell types, but the association between AngⅡ-JAK2-STAT3 pathway and renal interstitial fibrosis is uncertain.In this study, we explore the correlate between STAT3 signaling and renal interstitial fibrosis in cultured renal tubular epithelial cells.Western blot was used to evaluate the STAT3 acetylation and phosphorylation, as well as collagen Ⅳ, fibronectin and TGF-β1 protein levels.Transfection of recombinant palsmid vector carrying p300 gene was used to upregulate p300 expression.P300 siRNA was used to knockdown the p300 expression.The results showed that Ang Ⅱ increased STAT3 phosphorylation at Try705, TGF-β1, collagen Ⅳ and fibronectin expressions in renal tubular epithelial cells.These effects were blocked by curcumin, an inhibitor of JAK2 and p300/CBP.The inhibition of STAT3 acetylation at Lys685 by C646, p300siRNA and resveratrol decreased Ang Ⅱ-induced STAT3 phosphorylation at Try705, and TGF-31, collagen Ⅳ and fibronectin expressions.P300 up-regulated the basal level of STAT3 acetylation and enhanced Ang Ⅱ-induced STAT3 phosphorylation and relevant downstream protein expressions.These result suggested that the STAT3 acetylation is necessary for Ang Ⅱ-induced STAT3 phosphorylation and consequent extracellular matrix protein synthesis.
其他文献
Microgravity exposure may result in postflight orthostatic intolerance, which is associated with vascular remodeling.Nuclear factor κB (NF-κB) activation and increased inflammatory cytokines provide a
The aim of this study was to investigate whether decreased vasodilation in common carotid artery and abdominal aorta induced by hindlimb unloading (HU) is related with NADPH oxidase pathway.Arterial v
This study was designed to investigate the effects of 3-week simulated microgravity and intermittent artificial gravity on apoptosis of abdominal aorta smooth muscle cells (AASMCs) in rats.Hindlimb un
In vitro study has shown that activation of SK channels in the ventricular myocytes is responsible for the VF in failing ventricle.This study determined the role of SK channels in regulating VF in AMI
The calcium-activated chloride channels (CaCCs) are critical for many physiolo-gical functions.In cardiac tissues, CaCCs play important roles in the regulation of cardiac excitability.Although Anol (o
This study was to investigate whether Semen Cassiae extract reduces myocardial ischemia and reperfusion (MI/R) injury.Methods: The high-fat diet-fed streptozotocin (HFD-STZ) rat model (type 2 DM model
Background: Trauma induces not only primary heart injury but also secondary heart injury.Our previous study has demonstrated that mechanical trauma could cause cardiomyocyte apoptosis which contribute
To observe the changes of cardiac ALDH2 in diabetic rat, especially when diabetic rat was pretreated with ALDH2 activator ethanol (EtOH).The rats were divided into 5 groups: control, diabetic rat at f
Objectives: To testify whether exercise training (ET) could improve cardiac function in rats with myocardial infarction (MI) by promoting cardiomyocyte proliferation and stem cell chemotaxis.Methods:M
Expression of renal EP4 significantly increased in wild type mice after water deprivation, indicating that renal EP4 may participate in water homeostasis.Since global knockout of EP4 in mice induced l