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Urinary tract infections (UTI) are among the most common infectious diseases of humans and a potentially serious complication of hospitalized patients.The high frequency of infection results in huge annual economic costs,decreased workforce productivity and high patient morbidity.The commonest bacterial agent involved in causation of UTI is Escherichia coli,being the principal pathogen both in the community as well as in the hospital,but other rare and atypical pathogens are involved as well.While the bacteria will be identified correctly and rapidly,physicians could have the resistance profile of the microorganism and patients could be treated timely.The routine phenotypic identification of the rare and atypical microorganism is unsuccessful because of the weak and atypical biochemical reactions.