Inhibition of Coxsackievirus B3 propagation by RNA interference

来源 :2005 World DNA and Genome Day(2005中国(大连)国际DNA和基因组节大会) | 被引量 : 0次 | 上传用户：samallhu

【摘要】

：

【作者】

：

Steffen Schubert Heinz Zeichhardt Hans-Peter Grunert Denise Werk Diana Rothe Vanessa Lindig Volker A.Erdmann Jens Kurreck

【机构】

：

Free University Berlin, Institute for Chemistry(Biochemistry), Thielallee 63, D-14195, Berlin, Germa

【出处】

：

2005 World DNA and Genome Day(2005中国(大连)国际DNA和基因组节大会)

【发表日期】

：

2005年12期

下载到本地 , 更方便阅读

下载此文赞助VIP

声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架

论文部分内容阅读

　　Coxsackievirus B3 (CBV-3) is an enterovirus and one of the major cardial pathogens that may lead to dilated cardiomyopathy.RNA interference can be used to inhibit viral propagation for therapeutic purposes.Here,we present our efforts to design efficient small interering RNAs (siRNAs) against CBV-3.Initial experiments targeting the internal ribosome entry site in the 5 NTR were unsuccessful.This finding prompted us to investigate requirements for efficient siRNAs in more detail.Currently,most researchers focus on the thermodynamic properties of siRNAs themselves,when designing new siRNAs.

其他文献

Development of an Interspecies-specific Bioassay Using the Bovine Oocyte Model to Evaluate the Poten

Various mammalian species have been used for research on somatic cell nuclear transfer (SCNT).The bovine oocyte is an excellent model for establishing comparable biological parameters for SCNT.The aim

会议

Identification and Transcriptional Regulation of New Starch Modifying Enzymes in Aspergillus Niger b

1 Institute of Biology,Leiden University,2 Microbial Physiology Research Group,University of Groningen,Kerklaan 30,9751 NN,Haren,The Netherlands,3 Section Fungal Genomics,Wageningen University,Dreijen

会议

Screening, Cloning and Functional Analysis of Differentially Expressed New gene in Cervical Carcinom

In this study,a proto-oncogene and suppressor-tumor gene DNA microarray was utilized to analyze gene expression patterns in one primary cervical carcinoma.Total 11 genes (3.4%) showing consistently up

会议

Homo sapiens bladder cancer associated protein (BLCAP) geneCervical carcinoma

A novel Ensemble Decision Tree Approach to Mining Genes Coding ion Channels for Cardiopathy Subtype

Ion channels are critical for the normal physiological function of the organism.The functional abnormality of the ion channels may cause many disorders named channelopathy.Meanwhile,ion channels are o

会议

ion channeltransmembrane proteinensemble treefeature gene

The Comparison of Regulation of CyclinA Gene of a New Type HPV16 E7 Gene from Hubei Province of Chin

Objective:The comparison of carcinogenicity of HPV 16 HB variant E7 and HPV 16 WE7.Methods:To construct a luciferase reporter gene plasmid which was under the control of cyclinA promoter.HPV16 HB vari

会议

Regulation of the Rice Sucrose Transporter Gene 1 (OsSUT1) in Response to Sucrose in the Developing

Plant sucrose transporter is critical for sucrose across the plasma membrane,and it mediates the active distribution of sucrose in the plant growth and development processes.Rice sucrose transporter g

会议

in situ localizationricespatio-temporal expressionsugar contentsuspension ce

Cloning and Expression of an ADP-ribosylation Factor Gene OsARF3 in the Developing Spikelets of Oryz

The ADP-ribosylation factor (Arf) is a one of small GTP-binding protein,which performs as a major regulator of the vesicle biogenesis in intracellular traffic.Although the cloning and characteristics

会议

ADP-ribosylation factorRiceSmall GTP-binding protein

Simultaneous and Ultrarapid Determination of Reactive Oxygen Species and Reduced Glutathione by Micr

In this study,a simultaneous and ultrarapid microchip electrophoresis method coupled with laser-induced fluorescence (LIF) detection was established for the simultaneous determination of two important

会议

Isolation and Primer Designing of Microsatellite Marker in the Pacific Abalone(Haliotis discus hanna

Microsatellites have become a kind of popular marker types for genetic analysis,however,obtaining microsatellite sequences needs huge front work.In this experiment,we extracted and cured off total DNA

会议

Genes Regulated Following Prenatal Exposure to a MRI Magnetic Field

To seek genes regulated following prenatal exposure to a MRI magnetic field exposure,we first examined the effects of prenatal exposure to a 1.5T magnetic resonance image (MRI) magnetic field for 7 da

会议

Inhibition of Coxsackievirus B3 propagation by RNA interference

其他学术论文