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The Root Knot Nematode (RKN) Meloidogyne incognita is a widespread and polyphagous obligate asexual endoparasite of plants that causes serious and growing problems to agriculture.This lifestyle implies dramatical changes of plant cells into specialized feeding sites, which are induced by secreted proteins by the nematode, so-called parasitism effectors.An integrated approach of molecular techniques has been used to functionally characterize nematode parasitism proteins.