【摘 要】
：
Kaempferol, a natural compound frequently found in fruits and vegetables, has been reported to reduce the risk of ovarian cancer, but the mechanism is not completely understood.In this study, we tend
【机 构】
：
Natural Science Division Alderson-Broaddus College USA
【出 处】
：
BITs 3rd Annual World Cancer Congress-2012(2012第五届世界癌症大会)
论文部分内容阅读
Kaempferol, a natural compound frequently found in fruits and vegetables, has been reported to reduce the risk of ovarian cancer, but the mechanism is not completely understood.In this study, we tend to expand our understanding on how kaempferol regulates VEGF expression and angiogenesis in ovarian cancer cells.We timed VEGF secretion,and studied in vitro and in vivo angiogenesis by kaempferol treatment.Gene expression was examined by qRT-PCR,ELISA, western blotting, or luciferase assay, and pathways were examined by manipulating genetic components with plasmid or siRNA transfection.It was found that kaempferol time-dependently inhibited VEGF secretion, and suppressed in vitro and in vivo angiogenesis.Kaempferol down-regulated ERK phosphorelation as well as NFkB and cMyc expression, but promoted p21 expression.Examination of relationship between these genes suggested a novel ERKNFkB-cMyc-p21-VEGF pathway, which accounts for kaempferols angioprevention effects in ovarian cancer cells.This data supplements our comprehension of the mechanisms behind kaempferols biological influence in ovarian cancer cells, and better characterised kaempferol toward chemoprevention.
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