【摘 要】
：
Objective: To investigate the effect of TET on human gastric carcinoma cell lines SGC7901 and SGC7901/ADR, and its possible mechanism.Methods: Cultured SGC7901 and SGC7901/ADR were treated with differ
【机 构】
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Department of General Surgery,the Fourth Affiliated Hospital,Hebei Medical University,Shijiazhuang,C
【出 处】
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22nd Asia Pacific Cancer Conference(第22届亚太抗癌大会)
论文部分内容阅读
Objective: To investigate the effect of TET on human gastric carcinoma cell lines SGC7901 and SGC7901/ADR, and its possible mechanism.Methods: Cultured SGC7901 and SGC7901/ADR were treated with different TET concentrations (0.5, 1.0, 1.5, 2.0, and2.5 μg/mL).Inhibition rates were subsequently measured by MIT assay in vitro.Ihe expressions of ZNF139, MRP-1, MDR1,and GST-π were determined by RT-PCR.Results: Expressions of ZNF139, MRP-1, MDR1, and GST-πmRNA were higher in SGC7901/ADR than in SGC7901.The expressions of ZNF139, MRP-1, MDR1, and GST-π were efficiently downregulated in SGC7901/ADR cells (P＜0.05).Conclusion: TET could achieve MDR reversion in gastric cancer cells by downregulating the expression of ZNF139,MRP-1, MDR1, and GST-π.
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