Proteomic analysis of human Epithelial Lining Fluid for biomarker discovery in Chronic Obstructive P

• 作 者: L.Franciosi[1]N.Govorukhina[1]F.Fusetti[2]B.Poolman[2]M.Lodewijk[3]W.Timens[3]D.S.Postma[4]N.H.T.tenHacken[4]R.Bischoff[1]
• 机 构: University of Groningen, Department of Pharmacy, Analytical Biochemistry, Groningen, The Netherlands
• 出 处: 第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
• 发表日期: 2011年期

　　The development of new biomarkers to improve the diagnosis, prognosis and management of chronic obstructive pulmonary disease (COPD) is an urgent medical need.Epithelial Lining Fluid (ELF), collected during bronchoscopy from the main bronchi using microprobes, is a biofluid that is wellsuited to study pathophysiological processes in the lung, because it contains high concentrations of biologically active molecules.Proteomic analysis of ELF by 1-dimensional polyacrylamide gel electrophoresis followed by in-gel tryptic digestion and microfluidics-based liquid chromatography tandem mass spectrometry (chipLC-MS/MS) resulted in identification of 332 proteins.A comparative study of ELF from COPD patients and healthy controls using stable isotope labeling (iTRAQ(R)-8Plex) showed that the levels of lactotransferrin, high-mobility group box 1 protein, alpha 1-antichymotrypsin and cofilin-1 differed significantly in ELF from COPD patients and healthy controls.