【摘 要】
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Bocaviruses have a broad mammalian host range including humans, cows, pigs, gorillas, chimpanzees,Californian sea lions, dogs, cats, bats, and pine martens.Bocavirus, a genus of the Parvovirinae subfa
【机 构】
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College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University,Daqing
【出 处】
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中国畜牧兽医学会兽医内科与临床诊疗学分会第八届代表大会暨学术研讨会
论文部分内容阅读
Bocaviruses have a broad mammalian host range including humans, cows, pigs, gorillas, chimpanzees,Californian sea lions, dogs, cats, bats, and pine martens.Bocavirus, a genus of the Parvovirinae subfamily (Parvoviridae family), is characterized by the presence of a third major open reading frame (ORF) named NP1.The genome of bocaviruses is single-stranded (ss) DNA and approximately five kilobases in length, coding for non-structural and capsid proteins.In the past five years, several novel animal bocaviruses, from the pig, dog, and cat, have been discovered and characterized genetically.Therefore, a wide ranging molecular epidemiological investigation is necessary to improve understanding of bocavirus evolution.Recently, epidemics and apparent molecular evolution of human and porcine bocaviruses have been reported frequently in China, which has caused widespread concern.However, the genetic diversity of canine bocavirus (CBoV) strains circulating in China remains unclear.To trace the evolution of CBoV strains circulating in northeast China, 201 fecal samples from rectal swabs of diarrheic dogs collected from May 2014 to April 2015 were investigated using PCR targeting the partial NS1 gene (440 bp).Furthermore, phylogenetic analysis of the CBoV strains from northeast China was conducted, based on the partial NS1 gene.The results indicated that 15 of the 201 fecal samples (7.5%) were positive for CBoV;the partial NS1 genes of the 15 CBoV strains exhibited 83.1%-100% nucleotide identity, and 75.8%-100% amino acid identity.Phylogenetic analysis revealed that the 15 CBoV strains from China were divided into different subgroups of CBoV-2 when compared with strains of CBoV-2 from South Korea, the USA,Germany, and Hong Kong of China.The data revealed the presence of multiple novel genetic groups among the CBoV strains circulating in northeast China.
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