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Stressful events may adversely affect a childs physiological and psychological health.To evaluate the impact of stress on childrens well-being, it is important to develope valid and reliable stress assessment methods.Exhaled breath is a type of non-invasive biological sample, it can be used to define biomarkers (or breath prints) related to human biological process.In this article, a novel sampling and determination for exhaled breath has been developed and a preliminary exploration has been taken to make clear the relationship between the variation of the content of the VOCs and the psychological behavior of individuals.