Forward Genetic Screening for Regulators Involved in Cholesterol Synthesis Using Validation-based In

来源 :2014年第十二届全国脂质与脂蛋白学术会议 | 被引量 : 0次 | 上传用户：kakingka

【摘要】

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Somatic cell genetics is a powerful approach for unraveling the regulatory mechanism of cholesterol metabolism.However, it is difficult to identify the mutant g

【作者】

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蒋威唐静洁李伯良杨金波宋保亮

【出处】

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2014年第十二届全国脂质与脂蛋白学术会议

【发表日期】

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2014年10期

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Somatic cell genetics is a powerful approach for unraveling the regulatory mechanism of cholesterol metabolism.However, it is difficult to identify the mutant gene(s) due to cells are usually mutagenized chemically or physically.To identify important genes controlling cholesterol biosynthesis, an unbiased forward genetics approach named validation-based insertional mutagenesis (VBIM) system was used to isolate and characterize the 25-hydroxycholesterol (25-HiC)-resistant and SR-12813-resisitant mutants.

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Forward Genetic Screening for Regulators Involved in Cholesterol Synthesis Using Validation-based In

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