AngⅡ及受体在脑缺血大鼠脑内小胶质细胞中的表达变化

来源 :第九届海峡两岸心血管科学研讨会暨首届台湾南部国际心脑血管科学研讨会 | 被引量 : 0次 | 上传用户：lgs0519

【摘要】

：

【作者】

：

李娟娟查皓夏青青袁云李晓文郭小兵吴春云

【机构】

：

昆明医科大学人体解剖学与组织胚胎学系昆明 650500

【出处】

：

第九届海峡两岸心血管科学研讨会暨首届台湾南部国际心脑血管科学研讨会

【发表日期】

：

2013年4期

下载到本地 , 更方便阅读

下载此文赞助VIP

声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架

论文部分内容阅读

目的:观察成体大鼠局灶性脑缺血后,激活的小胶质细胞是否如同胚胎时期和生后早期大鼠脑内的阿米巴样小胶质细胞一样,再次表达血管紧张素Ⅱ(AngⅡ)及其受体(AT1和AT2受体),且表达变化规律如何;研究AngⅡ及其受体与脑缺血及激活的小胶质细胞三者之间的关系,探讨脑缺血的发病机理.方法:采用微创开颅法建立大鼠大脑中动脉闭塞(MCAO)模型,将81只成年雄性SD大鼠分为正常对照组(n=9),假手术组(n=9)和缺血组(随机分为缺血后3h、6h、12h,1d、2d、3d和1w组,每组n=9).应用免疫组织化学单标和荧光双标染色观察脑缺血后AngⅡ及其受体(AT1和AT2受体)在激活的小胶质细胞中的表达变化规律;用荧光定量PCR和Western blot技术检测脑缺血后AT1和AT2受体mRNA和蛋白的表达变化.

其他文献

壬基酚干扰17β-雌二醇对豚鼠缺血再灌注损伤心肌保护作用的研究

目的壬基酚(nonylphenol,NP)是环境内分泌干扰物的一种,因具有强大脂溶性而被广泛应用于工业、纺织业及生活用品中,目前对环境的污染甚为严重。众多研究证实NP长期积蓄于生物体内能对生殖系统、内分泌系统及心血管系统等产生不可忽视的毒性作用,而对其急性毒性作用的研究尚不明确。方法本研究首先将10-7 M NP和10-5 M NP直接灌流离体心脏,观察10-7 M NP和10-5 M NP对

会议

THE ACTIVATIONS OF COX-2/5-LO PATHWAYS IN BRAIN AFTER FOCAL CEREBRAL ISCHEMIA-REPERFUSION INJURY AND

OBJECTIVE: To investigate the activations cyclooxygenase-2 (COX-2)/5-1ipoxygenase (5-LO) pathways of arachidonic acid (AA) metabolism and the protective effects of 13-methyltetradecanoic acid (13-MTD)

会议

cerebral ischemia-reperfusion injury13-Methyltetradecanoic acidcyclooxygenase-

ANTIOXIDANT AND ANTI-HEAT ACTIVITIES OF FOUR SELECTIVE MEDICINAL PLANTS CONTAINING PHENOLIC AND FLAV

Hypoestes purpurea R.Brown (H.P.)(六角英), Procumbent Tridax (P.T.)(長柄菊), Emilia sonchifolia (E.S.)(一點紅) and Tithonia Diversifolia (T.D.)(五爪金英) are usually used in healthy tea.The healthy tea is associat

会议

INVESTIGATION OF ANTIOXIDATIVE STRESS IN VITRO AND WATER APPARENT DIFFUSION COEFFICIENT IN MRI ON RA

Spinal cord injury (SCI)-induced secondary oxidative stress associates with a clinical complication and high mortality.Treatments to improve the neurological outcome of secondary injury are considered

会议

SILENCING CIC-3 PREVENTS APOPTOSIS INDUCED BY PALMITATE IN PREADIPOCYTES THROUGH THE ER STRESS PATHW

ClC-3 chloride channel, plays an important role in the regulation of cell volume, proliferation and apoptosis.Preadipocytes of type 2 diabetes show a tendency to enhanced apoptosis.We hypothesized tha

会议

UROTENSIN Ⅱ INHIBITED THE PROLIFERATION OF CARDIAC SIDE POPULATION CELLS IN PRESSURE OVERLOAD MICE B

Cardiac side population cells (CSPs) are promising cell resource for the regeneration in diseased heart as intrinsic cardiac stem cells.However the relative low ratio of CSPs in the heart limited the

会议

Urotensin ⅡCSPsProliferationJNKLRP6

SITE-SPECIFIC HYPOMETHYLATION WITHIN PROMOTER CPG ISLAND OF PON1 WAS ASSOCIATED WITH BLEEDING EVENTS

To evaluate the role of the DNA methylation of the CpG island within the PON1 promoter on the clinical efficacy of the dual-antiplatelet therapy of aspirin and clopidogrel in patients with CAD after p

会议

SILENCING OF PELLINO1 IMPROVES POSTINFARCT CARDIAC DYSFUNCTION AND ATTENUATES LEFT VENTRICULAR REMOD

Aims: Pellinol is an evolutionally conserved immune regulator and participates in the regulation of activation of Toll-like receptor/intefleukin-1 receptor (TLR/IL-1R)-mediated signaling.TLR/IL-1R sig

会议

Myocardial infarctionPellinolTLR/IL-1RInflammationLeft ventricular remodelin

THE PROFIBROTIC ROLE OF MICRORNA-208B IN DIABETIC MYOCARDIAL FIBROSIS

Myocardial fibrosis plays a critical role in the process of diabetic cardiac remolding.MicroRNAs are endogenous, small non-coding RNAs that negatively regulate gene expression in diverse biological an

会议

ADIPOSE-DERIVED FACTOR C1Q/TNF-RELATED PROTEIN 6 PROMOTES VASCULAR SMOOTH MUSCLE CELL MIGRATION AND

OBJECTIVE: To investigate whether Clq/TNF-related protein (CTRP) 6 is involved in vascular smooth muscle cell (VSMC) migration and neointima formation in response to vascular injury.METHODS:Male Sprag

会议

AngⅡ及受体在脑缺血大鼠脑内小胶质细胞中的表达变化

其他学术论文