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Hypoestes purpurea R.Brown (H.P.)(六角英), Procumbent Tridax (P.T.)(長柄菊), Emilia sonchifolia (E.S.)(一點紅) and Tithonia Diversifolia (T.D.)(五爪金英) are usually used in healthy tea.The healthy tea is associated with "removing heat from the liver" (清肝解熱明目) in Taiwan.This study aims to determine the relationship between the antioxidant and anti-heat activities of the four herbs (including H.P., P.T., E.S.and T.D., and their total phenolic and flavonoid contents.