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Silk fibroin heavy chain is made up of hydrophilic and hydrophobic segments.During the dry-spinning process of silk fibroin aqueous solution,the interactions between the hydrophilic segments and water diminished gradually while those among the hydrophobic segments were improved for the silk fibroin assembly.However,the effect of water removal and Ca2+concentration was not well understood.In this paper,we constructed a dry-spinning apparatus mimicking the spinning duct of Bombyx mori to investigate how relative humidity and Ca2+concentration affect RSF assembly.The results of FTIR,WAXD and tensile test suggest that low relative humidity and higher Ca2+concentration promote RSF assembly.