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The stringent safety and efficacy criteria,along with the high cost of bringing new drug compounds into the market,are leading to an increased use of preclinical drug screening to eliminate false lead candidates.Though the use of animals in drug testing is necessary,it is an expensive and slow process and ethically controversial.Thus,much of the preclinical metabolism and toxicity evaluations rely on in vitro models,which can minimize the volume and cost of screening as well as the amount of compounds required [1].Hepatocytes constitute around 70%of the cellular population of the liver and play important roles in the activation of precursor molecules and the detoxification and inactivation of endogenous and exogenous substances.To address the challenges of a rapid function loss of primary hepatocytes,the coculture of hepatocytes with fibroblasts and endothelial cells was established on micropatterned fibrous scaffolds.The activities of phase Ⅰ and phase Ⅱ enzymes indicated a gradual increase for cocultured hepatocytes,and the metabolism testing on model drugs indicated that the scaled clearance rates for hepatocytes in the coculture system were significantly higher than those of other culture methods.