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Purpose: To evaluate the efficacy of a new automatic computer-aided detection (CAD) system for mass screening of retinal nerve fiber layer (RNFL) defects in a large population using fundus photographs.Methods: Among the fundus photographs of 1200 patients collected, a total of 2270 photographs appropriate for the analysis were tested.The photographs were first reviewed by two expert ophthalmologists for detection of RNFL defects (gold standard).