【摘 要】
：
To investigate whether the effect of maternal dietary protein on offspring lipid metabolism is mediated by miRNAs, fourteen Meishan sows were fed either low-protein (LP, half of standard protein level
【机 构】
：
Key Laboratory of Animal Physiology and Biochemistry,Nanjing Agricultural University,Nanjing 210095,
【出 处】
：
International Conference for Physiological Sciences 2012(201
论文部分内容阅读
To investigate whether the effect of maternal dietary protein on offspring lipid metabolism is mediated by miRNAs, fourteen Meishan sows were fed either low-protein (LP, half of standard protein level, n=7) or standard-protein (SP, n=7) diets throughout gestation and lactation.PPAR-γ and C/EBP-β protein expression were evaluated.The expression of miRNAs predicted to directly target PPAR-γ and C/EBP-β in the subcutaneous fat of offspring at weaning age were determined, and the functions of these potential miRNAs were verified.The results showed that piglet body weight and back fat thickness were significantly decreased in the LP group compared with the SP group (P<0.05).The protein level of PPAR-γ was significantly decreased, and C/EBP-β protein expression was also decreased, though not significantly (P=0.056), in the subcutaneous fat of the LP group.Furthermore,miRNA expression analysis showed that miR-130b, targeting the PPAR-γ 3-UTR, and miR-374b, targeting the C/EBP-β 3-UTR, were significantly increased in the LP group compared with the SP group;other candidate regulatory miRNAs were expressed similarly in both groups.Dual luciferase activity assay results indicated that miR-130b directly recognised and bound to the 3-UTR of PPAR-γ and thereby suppressed PPAR-γ gene expression.Similar results were found for miR-374b and the 3-UTR of C/EBP-β.The present study showed that miR-130b and miR-374b are involved in the effect of maternal dietary protein on offspring lipid metabolism in pigs.These results shed new light on our understanding of the maternal effect on offspring lipid deposition.
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