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Molecular methods, based on sequencing the region encoding the VP1 or complete P1 region, have enabled the rapid identification of new enterovirus serotypes.A strain of a new enterovirus type termed EV-B111 by the ICTV Picomavirus Study Group, which isolated from stool samples from an acute flaccid paralysis patient in China, is described.Nucleotide sequence encoding the complete P1 capsid protein is less than 65% identity to the homologous sequences of the recognized human enterovirus serotypes, and the deduced amino acid sequence of the complete P1 region are less than 76% identical to those of the recognized serotypes.