【摘 要】
：
杆状病毒在其复制周期中产生两种结构和功能不同的病毒体：芽殖型病毒体(budded virus,BV)和包涵体源性病毒体(occlusion derived virus,ODV).其基因表达具有时序性,可以区
【机 构】
：
华中师范大学生命科学学院,遗传调控与整合生物学湖北省重点实验室
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杆状病毒在其复制周期中产生两种结构和功能不同的病毒体：芽殖型病毒体(budded virus,BV)和包涵体源性病毒体(occlusion derived virus,ODV).其基因表达具有时序性,可以区分为：早期,晚期和极晚期表达.37个基因存在于所有已完成测序的杆状病毒基因组中,称为核心基因.e25(odv-e25)是其核心基因之一；晚期表达.e25的编码产物存在于BV和ODV被膜,但在病毒复制中的功能尚未明了.本文利用BAC-to-BAC系统和λ-Red同源重组技术构建了苜蓿银纹夜蛾核型多角体病毒(Autographa californica multiple nucleopolyhedrovirus,AcMNPV) e25缺失、早表达、超量表达以及序列突变体,分析e25突变对病毒形态发生和基因表达的影响,分析了E25蛋白与宿主蛋白的相互作用.
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