Development of a simple and rapid sample pre-treatment method for the content determination of flavo

来源 :第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会 | 被引量 : 0次 | 上传用户：bluelink

【摘要】

：

【作者】

：

Dong Pei Shengrong Ning Yewei Liu Mingrui Shi Duolong Di

【机构】

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Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine

【出处】

：

第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会

【发表日期】

：

2011年3期

【关键词】

：

High shear dispersing emulsifier HPLC Flavonoids Bee pollen of Brassica napus L

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　　In this paper, a simple and rapid sample pre-treatment method, High Shear Dispersing Emulsifier(HSDE) extraction, had been developed for the content determination of flavonoids in bee pollen by high performance liquid chromatography(HPLC) and compared with ultrasonic extraction and immersion extraction.The results shown that HSDE had higher efficiency on breaking the cell wall and extracting flavonoid compounds of the pollen than other two methods.The optimized HSDE conditions were as follows: shearing time was 3 min, shearing Speed was 19000 r/min, ratio of liquor to material was 20∶1 (v/w).The wall-broken rate of bee pollen was higher than 98% with this treatment.The detection of pre-treated samples was carried out on HPLC coupled with a diode array detector (DAD).

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