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Five anthraquinones including aurantio-obtusin,1-desmethylaurantio-obtusin,chryso-obtusin,obtusin,and 1-desmethylchryso-obtusin were isolated and purified by high-speed counter-current chromatography (HSCCC) and semi-preparative high-performance liquid chromatography (HPLC) from Cassia tora L.