【摘 要】
：
Factor Ⅷ (FⅧ) is a large protein that is essential for normal hemostasis.Activated factor Ⅷ (FⅢa) serves a cofactor function,increasing the catalytic rate of the serine protease factor Ⅸa (FⅨa) by fou
【机 构】
：
Puget Sound Blood Center University of Washington USA
【出 处】
：
2008中国深圳蛋白质和多肽科学大会
论文部分内容阅读
Factor Ⅷ (FⅧ) is a large protein that is essential for normal hemostasis.Activated factor Ⅷ (FⅢa) serves a cofactor function,increasing the catalytic rate of the serine protease factor Ⅸa (FⅨa) by four orders of magnitude.Deficiencies in FⅧ lead to the bleeding disorder hemophilia A,which affects one in 5000 males worldwide.Hemophilia A may be treated by infusions with recombinant or plasma-derived FⅧ.Unfortunately,the FⅧ protein is highly immunogenic,and approximately one quarter of patients who receive these infusions develop an immune response in which anti-FⅧ antibodies block its procoagulant function.Most of the inhibitory IgGs prevent FⅧa binding to phospholipid membranes or FIXa,and they bind primarily to the A2 and C2 domains of FⅧ.
其他文献
Multiple analytical technologies were employed to analyze various variants and/or impurities ofmonoclonal antibodies.Charge variants such as sialated species,deamidated species and species with un-pro
Chromatography is the standard technology for purification of protein-based drugs.Instrumental to good process economy at manufacturing scale is the re-use of resins.However,clinical material is commo
High specificity and high atTmity monoclonal antibodies (mAb) are crucial both for the discovery and validation of biomarkers for human health and disease and as clinical diagnostic and therapeutic ag
Recent advances in structural biology have made possible the high throughput structure determination of proteins,which offers the basis for rational drug design and biomolecular engineering.In these a
Label-free interaction analysis based on surface plasmon resonance (SPR) allows detailed investigations of protein binding events and has therefore become a standard tool for academic research and dru
11-beta hydroxysteroid dehydrogenase type Ⅰ (11β-HSD 1) is an ER-membrane associated short-chain reductase that catalyzes the conversion of cortisone to cortisol,a stress hormone associated with vario
Elongation factors EF-Tu,members of the family of GTP-binding proteins,belong to key elements of protein synthetic machinery in all bacteria.They are responsible for the delivery of amino acids,buildi
Low-affinity protein-protein interactions (PPI) between domains of modular proteins and short,solvent-exposed peptide sequences within their binding partners play an essential role in intracellular si
Cell membranes are laterally organized into functionally discrete domains that include the cholesterol-dependent membrane "rafts," and which function in regulating the association of membrane proteins
Terahertz time-domain spectroscopy (THz-TDS) is a technique based on femtosecond,short pulse lasers,which enables to investigate both the frequency-dependent absorption coefficients and refractive ind