【摘 要】
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Recent advances in structural biology have made possible the high throughput structure determination of proteins,which offers the basis for rational drug design and biomolecular engineering.In these a
【机 构】
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Institute for Molecular Science Nagoya City University Japan
【出 处】
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2008中国深圳蛋白质和多肽科学大会
论文部分内容阅读
Recent advances in structural biology have made possible the high throughput structure determination of proteins,which offers the basis for rational drug design and biomolecular engineering.In these approaches,recombinant proteins subjected to NMR and X-ray crystallographic analyses are produced by using bacterial or cell-free expression systems and therefore do not possess sugar chains.However,many of the proteins in the living systems express sugar chains,which play crucial roles in regulation of glycoprotein functions and even in glycoprotein-fate determination in cells.To elucidate the underlying mechanisms of the sugar functions,we have been developing a systematic method for structural glycobiology by combined use of a 920 MHz ultra-high field NMR spectroscopy and multi-dimensional HPLC techniques for glycosylation profiling and sugar library construction.We have also developed GALAXY,a web application for profiling of N-linked oligosaccharides (http://www.glycoanalysis.info/ENG/index.html).
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