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SSR (simple sequence repeats) also known as microstatellites, exist extensively in genomes and show high levels of polymorphism.The thorough scanning of the location and distribution of SSR in genome would facilitate their continued use as a molecular marker and contribute to a general understanding of the genome organization, phenotypic diversity and the microsatellite evolution in genomes.Thus we characterized the SSR pattern in the genome ofBoletus edulis and compared it with SSR patterns found in three other edible fungal genomes by the bioinformatics method.