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We have used bulked segregant analysis to screen the strain-specific DNA marker associated psychrophilic strain of Pleurotus eryngii.Bulked genomic DNAs of Pleurotus eryngii were amplified by randomly amplified polymorphic DNA (RAPD) using OP-A, OP-B, OP-L, OP-P, OP-R and OP-S primers to screen the strain-specific DNA marker.