First report of genotyping of Toxoplasma gondii in wildlife on the Qinghai:Tibet Plateau,China

• 作 者: 张晓轩 娄忠子 黄思扬 周东辉 贾万忠 苏春雷 来兴全
• 机 构: 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室,兰州730046;延边大学农学院,延吉133000
• 出 处: 中国畜牧兽医学会家畜寄生虫学分会第七次代表大会暨第十二次学术研讨会
• 发表日期: 2013年7期

　　The distribution of genetic diversity ofToxoplasma gondii in wildlife is of interest to understand the transmission of this parasite in the environment.Limited information on T.gondii genotypes has been reported in wildlife in China.Using PCR and multilocous polymerase chain reaction:restriction fragment length polymo rphism (PCR:RFLP) technology,we conducted a survey to examine genetic diversity of this parasite in wildlife on the Qinghai:Tibet Plateau,China.In total,183 brain tissues of different wild animals,including 48 Qinghai vole (Microtus fuscus),101 Plateau Pika (Ochotona curzoniae) and 34 Tibetan Ground:tit (Pseudopodoces hum ilis),were tested for T.gondii infection.11 of which were found to be positive for the T.gondii B 1 gene by PCR amplification.These positive DNA samples were typed at 10 genetic markers,including 9 nuclear loci (SAG 1,5:and 3:SAG2,alternative SAG2,BTUB,GRA6,L358,PK1,c22:8,c29:2),and an apicoplast locus Apico.Six were successfully genotyped at eight or more genetic loci,and were grouped to three distinct genotypes.Fo ur samples belonged to ToxoDB Genotype #10 and the other two samples were identified as two new genotypes (http://toxodb.org/toxo/).The results show that there is a potential risk for the transmission of this parasite thro ugh the wildlife in this region.To our knowledge,this is the ?rst report of genetic typing of T.gondii isolates in wildlife on the Qinghai:Tibet Plateau,China.