Prevalence and Genetic Characterization of Toxoplasma gondii in House Sparrows (Passer domesticus) i

• 作 者: 从伟 黄思扬 周东辉 张晓轩 张念章 赵权 朱兴全
• 机 构: 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室,兰州 730046;吉林农业大学动物科技学院,长春,130118
• 出 处: 中国畜牧兽医学会家畜寄生虫学分会第七次代表大会暨第十二次学术研讨会
• 发表日期: 2013年7期

　　Toxoplasma gondii is an important protozoan parasite that infects most species of warm:blooded animals worldwide,including humans and birds.The prevalence ofT.gondii infection in birds has epidemiological significance because birds are indeed considered as a good indicator of environmental contamination by T.gondii oocysts.In this study,the prevalence ofT.gondii in 313 house sparrows in Lanzhou,northwestern China was assayed by the modified agglutination test (MAT).Antibodies to Toxoplasma gondii were found in 39 (12.46%) of 313 samples (MAT titer≥ 1:5).Tissues of heart,brain and lung from the 39 seropositive house sparrows were tested for T.gondii DNA,11 of which were found to be positive for the T.gondii B 1 gene by PCR amplification.These positive DNA samples were typed at 9 genetic markers,including 8 nuclear loci,i.e.,SAG 1,5: and 3: SAG2,alternative SAG2,SAG3,GRA6,L358,PK1,c22:8 and an apicoplast locus Apico.Of them,4 isolates were genotyped with complete data for all loci,and two genotypes (Type Ⅱ variants: ToxoDB #3 and a new genotype) were identified.These results show that there is a potential risk for human infection with T.gondii in this region.To our knowledge,this is the ?rst report ofT.gondii seroprevalence in house sparrows in China.