【摘 要】
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A major regulatory mechanism evolved by microorganisms to combat stress is the regulation mediated by (p)ppGpp (the stringent response molecule),synthesized and hydrolyzed by Rel proteins.These are di
【机 构】
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Indian Institute of Technology India
【出 处】
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2008中国深圳蛋白质和多肽科学大会
论文部分内容阅读
A major regulatory mechanism evolved by microorganisms to combat stress is the regulation mediated by (p)ppGpp (the stringent response molecule),synthesized and hydrolyzed by Rel proteins.These are divided into bifunctional and monofunctional proteins based on the presence or absence of the hydrolysis activity.While these proteins require Mg2+ for (p)ppGpp synthesis,high Mg2+ was shown to inhibit this reaction in bifunetional Rel proteins from M.tuberculosis and S.equisimilis.This is not a characteristic feature in enzymes that use a dual-metal-ion mechanism,like DNA polymerases that are known to carry out a similar pyrophosphate transfer reaction.
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