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Objective To observe the expression of fatty acid synthase (FASN) in alcohol-induced liver injury in mice, and investigate the possible mechanism of EGFR in the process.Methods Primary mice hepatocytes were cultured conventionally.Four groups included normal group (saline group), ethanol group, ethanol plus EGFR tyrosine kinase inhibitor (Gefitinib) group and Gefitinib alone group were set up randomly.Total RNA and protein from liver cells were extracted, and real time-PCR and western-blot were applied to measure the gene and protein expression of FASN, Sterol regulatory element binding proteins (SREBP-1c) and EGFR.