【摘 要】
：
Haploid cells are amenable for genetic analysis.Recent success in the derivation of mouse haploid embryonic stem cells (haESCs) via parthenogenesishas enabled genetic screening in mammalian cells.Howe
【机 构】
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Shanghai Institute of Biochemistry and Cell Biology , CAS
【出 处】
：
第十五届亚太分子生物学网络组织年会
论文部分内容阅读
Haploid cells are amenable for genetic analysis.Recent success in the derivation of mouse haploid embryonic stem cells (haESCs) via parthenogenesishas enabled genetic screening in mammalian cells.However,successful generation of live animals from these haESCs,which is needed to extend the genetic analysis to the organism level,has not been achieved.Here,we report the derivation of haESCs from androgenetic blastocysts.These cells,designated as AG-haESCs,partially maintain paternal imprints,express classical ESC pluripotency markers.and contribute to various tissues,including the germline,upon injection into diploid blastocysts.Strikingly,live mice can be obtained upon injection of AG-haESCs into MII oocytes,and these mice bear haESCcarried genetic traits and develop into fertile adults.Furthermore,gene targeting via homologous recombination is feasible in the AG-haESCs.Our results demonstrate that AG-haESCs can be used as a genetically tractable fertilization agent for the production of live animals via injection into oocytes.
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